Dear: Mirajkar

Possibly is a short time centrifugation, because the manufacturer recomended 10 minute.

I would like more information about your patient and this  influence in sample..

Best regards

Dear Billy,

You are right  I  am centrifuging for 10 minutes only as per protocol. Even after 10 minutes there is no separation .This problem I am encountering only in this patient. Only difference, the blood sample was frozen at -80 C for a week. 

Patient is healthy participant . Only information I know about patient  available is patient  takes  supplements  like  omega fatty acids, vitamins, lots of nuts etc.

Dear: Mirajkar 

It´s interesting this case but i think possibly interference was frozen... do you report this case.

I have read protocols of various laboratories.

It is time sensitive. Separation must be performed within few hours.

Storage of blood sample should be at room temperature or 4 C.

Be sure to draw the full volume to ensure the correct blood-to anticoagulant (EDTA) ratio.

The EDTA tube used for plasma and buffy coat preparation should NOT be the first blood sample.

Storage of plasma should be carried out at -80 C.

Dear Mushtaq

At any case you should be able to see the plasma. We have a number of blood samples that on just storage we can see and separate plasma except in one case where we have this problem.

It is quite interesting and unique. Is it possible that donor again donates blood and you perform separation.

Dear Mirajkar,

I think the frozen whole blood had lysed the red blood cells and mixed with the plasma, therefore, you could not obtain the straw colour of plasma.

It is is a short time centrifugation, so the manufacturer recomended 10 minute.

Abdur Mirajkar

I agree with Ai Mun Cheong ,

Thawing of red blood cells leads to their hemolysis. Therefore, after thawing, your sample is a cells-free mixture of hemolysate and plasma.

It is necessary to carry out the separation of cells before freezing.

I too facing the same problem, there is a Zero sedimentation of RBSs in one sample on the other hand, on another sample, plasma is easily separated, all condition/ protocol is same.

We have the same situation in some cases not in one. And they are not frozen, but the the samples for CHD child.

we just encountered the same situation in an entire family, without any logical explanation

I encountered this kind of blood sample with PCV cases.