Why is the Hoechst 33258 dye for DNA quantification using jasco fluorometer giving me no stable reading but goes up and down?

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Hayder Obayes Hashim

well there are several reasons such as

1- if your dna sample have a large particles in this case centrifuge your sample (12000 g) and take the supernatant to measure

2- check your device may be it need a calibration or fixing

3- the input voltage can led to this too.

check these and there more .

Shaimaa Hassan

Dr hayder thanks for ur answer but i wanna tell u that I use NaCl & Tris &edeta of normal analytical grade not molecular biology grade could it affect the result or no?