Hi,

I'm using the Quiagen RNase-free Dnase and the RNeasy mini kit to clean up and DNase treat my RNA samples (extracted from pure microbial cultures). I get around 500-1000 mg/μl of RNA after the extraction and the on-column DNase treatment (4 hrs, 37 degrees celsius), but I need to clean up my RNA once using the kit before performing the treatment for the DNase-treatment to be effective. I've opened up a new kit recently and it looks like my RNA is degrading completely after the clean-up and Dnase-treatment. I've also tried adding the Promega RNasin to prevent this, but it doesn't seem to work. I've tried these steps a few times before and they seemed to work. I don't know it I am doing something wrong or if there is a possibility that the kit might be faulty. Need suggestions!