The fusion protein I´m working with stains well with coomassie, but not with silver. After the fusion hydrolysis, the digestion subproducts are stained both with silver and coomassie, but I can´t detect the untreated fusion with silver.
Debaki Ranjan Howlader
Are all the silver staining steps being followed thoroughly and properly? What about all the reagents that you need for silver staining? I hope they're in good condition. Is it working in case of other samples too?
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Martin Somoza
Hi Amanda, it's possible that your protein is binding the silver ions and preventing the reaction. This binding activity maybe it's primary structure dependent, so digestion products do stain.
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