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Questions related from Martin Somoza
Hello, I am planning to do a cytokine immunoflourescence in spleen tissue slices. In bibliography most methods do not permeabilize cells, thereby detecting extracellular protein (associated to...
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Hi, I am looking for an in vitro protocol of T-dependent B cell responses. As far as i could see, there are several protocols for T-independent responses via stimulation with LPS, anti-IgM,...
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Hi, I´ve been staining SDS page gels using the silver reduction technique. The main goal is to cut off differential bands (between treatments) from the gel and analyze it by MS. After purification...
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Hi, Yesterday i was purifying biotinylated molecules with a neutravidin polyacrylamide resin and after incubation with cell lysate, the resin looked "aggregated" instead of a conventional resin...
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Hi, I'm having trouble trying to stain mouse IL-4 from Th2 cells polarized in vitro.I've been purifying CD4+ cells from BABL/c spleens with magnetic beads and culturing them in CD3/CD28 pre-coated...
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