Hello,
In my lab course we amplified plasmids but after PCR one of my products turned out to be a bright pink and half the volume. I believe the tube may have opened so some of the contents may have evaporated but I am not sure why the product is pink. Any suggestions?
Abhijeet Singh
It might be due the chemical contamination during the preparation step. There might me something in your pipette tip or PCR tube etc. which might have caused the colour.
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Frederic Lepretre
Hi Magda,
the only cause I can see for this pink color is the addition of BET during one step. if right, take care because BET is toxic, you'll nee to eliminate all stuffs by the right protocol.
fred
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