Why is my DH5alpha lag phase tooooo long in defined medium?

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Chinaza Godswill Awuchi

Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4475895/

Also check https://aem.asm.org/content/85/14/e00486-19

Jim C Philp

If it is valid for you to use a larger starter inoculum from a rapidly growing culture then this could help. It would have to be from the same defined medium to prevent carry-over of other nutrients.