Raviprasadh Rajasekaran

5 Questions 3 Answers 0 Followers

Questions related from Raviprasadh Rajasekaran

Why is my C elegans not growing in the NGM plate when transferred from the live mother plates?

Hi All, I recieved C elegans from CGC, US. They were very active after I added few drops of cultured OP50 bacteria. On observing under the microscope, I could see lots of active small and big C...

29 March 2021 2,086 0 View

Is there any possibility that mice with increased B-oxidation, can still develop fatty liver disease upon aging?

Dear All, I am studying the mechanism of a protein localized in the mitochondria of liver in a mice knocked out for my specific protein. The liver has increased lipid droplets in aged mice. Also...

11 January 2016 8,445 3 View

Are there any guidelines regarding C terminal FLAG fusion protein purification from cell line for N terminal sequencing?

Dear Researchers, I am working on mitochondrial localized protein, I hereby expressed it in HEK cell line with C terminal FLAG and confirmed its targeting. Now I want to purify to send it to be...

31 July 2015 2,038 3 View

Can anyone please give suggestions for why my restriction digestion gives a higher band than expected?

Experiment detail: I am digesting the pCMV6 Neo Vector of 5838bp and pCMV XL5 of 4700bp with newly bought Not1 enzyme from Promega in separate reactions for 2h at 37 C followed by heat...

01 May 2014 9,992 8 View

Can anyone suggest the reason for E9 cell death after 32 hours transfection?

I am transfecting E9 cells with K44a with pcDNA 3.1 backbone. The transfection reagent is PEI, the purity of the plasmid is 1.9 and conc is 340 ng/ul. I used 0.5 and 1 ug conc of Plasmid and 1.5...

11 October 2012 2,022 5 View