I started with partial purification of amylase using ammonium precipitation technique.While going for step by step saturation the recovery of protein was less than 10% of the crude amylase.So i started with 20ml crude enzyme direct 100% saturation.The precipitates were dissolved in 500ul of phosphate buffer and poured into dialysis bag,dialysis bag was dipped in phosphate buffer.After 24 hours there were crystals in the beaker contain buffer. I changed the buffer and kept for another 24 hours yet another day i see the crystals in the beaker This process continued for a week. After a week also some crystals were seen in the beaker but i startet with the protein estimation. I was expecting atleast 80% recovery but again i got only 12% recovery.....the quantity of protein had also increased from 500ul to 1ml