Himanshu Sharma

6 Questions 8 Answers 0 Followers

Questions related from Himanshu Sharma

Why is it that some crude protein are less precipitated with ammonium sulphate salts but readily precipitated with prechilled ethanol ?

I have been working on alpha amylase and tried precipitating it with ammonium sulphate salt. With ammonium sulphate salt i do get precipitates but they are flaky, float in the supernatant and...

07 July 2017 7,160 5 View

Which sephadex to use in sixe exclusion to seperate 66 KDa protein?

I need to seperate 66 Kda protein from the other proteins of lesser molecular weight but are closely placed on native page. i plan to use sephadex for size exclusion but i am not sure about what...

08 August 2016 2,938 8 View

How to prepare glucose, mannose, sucrose, lactose, starch, ribulose, trehalose, mannose, sample for thin layer chromatography?

I need to prepare standard with sugars for which i need to know the solvent in which i should dissolve sugars if i will be using chloroform:acetone:water 6:7:1 ratio as solvent and spray of...

06 June 2016 7,637 2 View

How to speed up the destaining of sds page?

i am using sds page to know the molecular weight of protein i am working with. i have used coomassie blue to stain the gel. Its been over a week now that i am changing the destaining solution...

12 December 2015 6,558 5 View

Which protein membrane centrifuge tubes should be used to concentrate the protein of 66 KDa?

i have precipitated protein using ethanol........do i need to concentrate the protein before i start chromatography techniques......... if yes then what centrifuge membrane tubes should i use for...

12 December 2015 3,526 6 View

Why is my ammonium sulphate protein precipitation not working properly?

I started with partial purification of amylase using ammonium precipitation technique.While going for step by step saturation the recovery of protein was less than 10% of the crude amylase.So i...

10 October 2015 6,617 17 View