When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Checking for overfitting in niche models?

In niche models, overfitting has been traditionally assessed by large difference between training and test AUC or other model performance metrics. Are there other methods to assess model...

07 October 2020 9,060 3 View

How do we balance the argument on the effect of hydroxychloroquine in Covid-19 prophylaxis and treatment?

Hydroxyl-chloroquine has been listed as a potential drug in the management of Covid-19. The effect of this drug in Covid-19 prophylaxis and management is trending on social media. The Doctors...

29 July 2020 2,552 4 View

Positive value in NCT real-time PCR?

Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...

07 March 2020 3,953 4 View

Can anyone help with a detailed assay procedure for the spectrophotometric measurement of protein carbonyls in tissues?

Protein carbonyls are important compounds to measure progression of oxidative stress in tissues. Available articles online do not have details about how to do final estimation/calculation of the...

06 February 2020 2,897 8 View

What significance is the following?

What is the significance of the following solutions being used as rodent"s organ fixatives, between normal saline, citrate buffer and sucrose?

09 July 2019 5,580 1 View

Suction pump of the Rotary evaporator?

Can someone explain why the suction pump of the rotary evaporator sucks solvent from the condensation chamber, also pumping out engine oil when it's being switched off and perhaps the solution to...

19 June 2019 3,530 13 View

Where can i purchase tail cuff device?

Anyone with the idea of where to buy tail cuff device for measuring the blood pressure of rats. Appreciation in anticipation. Fasan

10 April 2019 2,700 2 View

Defat before phenolic rich fraction extraction?

06 April 2019 2,866 8 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Is CXCR5 on T cells affected after a cell sorting?

I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...

15 February 2021 8,034 7 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Are there well-established collaborative sorting tasks/datasets?

Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...

31 January 2021 4,979 3 View

Is there a technique to store fixed cells after flow for later RNA analysis?

I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...

21 January 2021 9,722 3 View

Are Signal Peptide and Transit Peptide different? And what are their functions?

I want to learn about mutation in transit peptide. Can mutation in transit peptide affect the function of the protein?

20 January 2021 396 3 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

Metabolomics Data Normalisation?

Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...

04 January 2021 8,773 5 View