Why is it recommended to add sample buffer to empty wells in SDS PAGE?

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Omar Gonzalez-Ortega

To reduce lateral diffusion

Mert Sudagidan

Dear Shilpa, the presence of charged molecules especially salts in your samples may affect the mobility of your proteins in SDS-PAGE gel.

Best regards.

Gustavo Zardeneta

It is recommended but it is not necessary, especially if you avoid using the well on either end of the gel. As Omar states, it helps avoid lateral diffusion which may create a smile but I hardly ever see this problem.