Can we expect a reduction in the population of particles in SEM images in the form of particles and after making their films?

I took scanning electron microscope images of my samples in powder form and after coating their films. In powder form, a large number of particles can be seen however, in the films only a few...

06 July 2019 1,956 4 View

Which solvent is suitable for analysing the UV Vis spectra of a polydiacetylene compound?

I want to study UV Vis spectra of a polydiacetylene based compound. It is a polymer and is not soluble in most of the solvents. i tried dissolving it in ethanol, butanol, chloroform, DCM, THF, DMS...

09 October 2017 2,579 3 View

What makes some of the diacetylene compounds highly UV sensitive?

05 June 2017 2,994 4 View

How is shift in Raman peaks of polydiacetylenes related to change in their conjugation length?

The shift in Raman peaks implies increase or decrease of conjugation length of polydiacetylenes?

03 April 2017 1,698 3 View

Does the nanoparticle form of monomers increase the chain length during radiation induced polymerization?

During radiation induced polymerization of diacetylenes will it be useful to prepare monomers in nano range to increase the chain length.

11 December 2016 6,764 4 View

How is excitation energy transferred from the side group to the triple bond in diacetylene?

Diacetylenes are a conjugated system consisting of two triple bonds. The solid state polymerization of diacetylenes proceeds as 1,4-addition to the conjugated triple bonds and gives rise to...

06 July 2016 8,663 1 View

Can a hygroscopic chemical be insoluble in water?

Why?

04 May 2016 2,965 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View