Can we expect a reduction in the population of particles in SEM images in the form of particles and after making their films?

I took scanning electron microscope images of my samples in powder form and after coating their films. In powder form, a large number of particles can be seen however, in the films only a few...

06 July 2019 1,956 4 View

Which solvent is suitable for analysing the UV Vis spectra of a polydiacetylene compound?

I want to study UV Vis spectra of a polydiacetylene based compound. It is a polymer and is not soluble in most of the solvents. i tried dissolving it in ethanol, butanol, chloroform, DCM, THF, DMS...

09 October 2017 2,579 3 View

What makes some of the diacetylene compounds highly UV sensitive?

05 June 2017 2,994 4 View

Why is it important to purify a polydiacetylene compound before use? What are the methods to purify them?

If we use 10,12 pentacosadiynoic acid for a reaction, is it mandatory to purify it before use? How can it be purified, without loosing its color sensitivity?

02 March 2017 1,724 1 View

Does the nanoparticle form of monomers increase the chain length during radiation induced polymerization?

During radiation induced polymerization of diacetylenes will it be useful to prepare monomers in nano range to increase the chain length.

11 December 2016 6,764 4 View

How is excitation energy transferred from the side group to the triple bond in diacetylene?

Diacetylenes are a conjugated system consisting of two triple bonds. The solid state polymerization of diacetylenes proceeds as 1,4-addition to the conjugated triple bonds and gives rise to...

06 July 2016 8,663 1 View

Can a hygroscopic chemical be insoluble in water?

Why?

04 May 2016 2,965 0 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View