Insects and COVID-19: a possible contaminant?

Dear colegues, What do you think about this? Can insects or others arthropods transmit the virus (directly or indirectly)?

19 April 2020 7,391 53 View

Forests Pests: increased damage by climate change?

Dear researchers, what is your opinion about climate changes and forests pests? there is an increase or reduction of damage caused by insects and mites on trees?

29 February 2020 2,434 17 View

Immunohistochemistry antibodies for cat immune cells?

I am currently getting desperate trying to find antibodies that would work in parafin-embeded tissue from the cat. I am aiming to stain different immune cells and cannot find a specific primary...

25 July 2019 1,287 4 View

How much dummy variables can you use in a regression without getting the constant term to be huge and significant?

When using multi dummy variables, the constant term gets bigger in absolute value, and significant in pvalue.... and most importantly, you just can't make sense out of it, to have a clear...

20 November 2018 6,699 9 View

Can anyone assist me in plotting the Fourier series shown in the attached picture using Matlab code?

It's the signal of the first picture with the format of the second picture. I would be very grateful.

11 December 2017 1,522 2 View

Image denoising using lagrange multipliers; Optimal multiplier question.

My question goes from the explained in the attached document. It's about image denoising using regularization, with constraints. I used the Lagrange multipliers formulation, but, by obtaining the...

16 May 2017 761 2 View

Freezing BHK cells

17 October 2016 5,034 1 View

How can multimedia enhance learning process?

Hi, I'm currently working on a research to find out how can multimedia (resources and environments) enhance learning process, namely among young learners (elementary school). It would be helpful...

31 August 2016 3,151 32 View

Is it possible to reliably predict plant miRNA targets using solely blastn?

Hi everyone! Although I am aware of the existence of several tools for predicting miRNA targets, I would like to know if it is possible to use only blastn to this end in case of plant miRNAs. As...

24 August 2015 8,262 4 View

Should I concatenate phased nuclear partitions with a mitochondrial one?

To generate a concatenated matrix for phylogenetic analyses among phased nuclear genes and mitochondrial, would you duplicate the mt haplotypes, or randomly select one allele of each nuclear...

30 April 2015 8,338 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View