Anthocyanin and Flavanoids are useful secondary metabolites from microbes and plants. These are separated using various solvents, especially acidified methanol solution. This is an industrial process which makes use of diffusion of these metabolites across cell membrane. Why is acidified methanol preferred to other solvents?
Alexander Galushko
Some anthocyanins could be mono- or di-acylated and stability of these compounds strongly depends on pH, temperature and others. It is suggested that "slight" acidification of solvents prevents degradation of these metabolites (Fan et al 2008 Optimizing conditions for anthocyanins extraction from purple sweet
potato...)
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