Srivatsava Viswanadha

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Questions related from Srivatsava Viswanadha

What is the shelf-life of stock solutions (in DMSO) of PD0325901 and CHIR99021 when stored at -20C ?

Stock solutions of MEK/ERK inhibitor - PD0325901 and GSK3 inhibitor - CHIR99021 were prepared in DMSO and stored as aliquots in tightly sealed eppendorfs. For how long can these aliquots be stored...

03 August 2020 1,460 4 View

Which class of compounds is the best to achieve senescence in cancer cells?

Senescence, defined as the arrest of growth of mammalian cells and prevents unlimited proliferation is of considerable interest in cancer treatment.

24 March 2014 10,035 6 View

Can someone name the important pro apoptotic response and the various assays for their quantification?

Time staggered treatment of EGFR inhibitors and genotoxic drugs result in enhanced cancer cell death. Synergistic relation between the drug can be studied by varying the time gap between their...

28 September 2013 3,888 4 View

Which is a better assay among Alamar blue and MTT?

These assays are used to test cytotoxicity and cytostatic activity

16 September 2013 8,592 13 View

What happens to ATP synthesis when the Electron Transport Chain (ETC) is inhibited at Cytochrome B (Cyt b) by Antimycin A

Antimycin A inhibits ETC at the "cytochrome b" site in complex 3 . This regulation leads to the complete halt of electron transport in mitochondria . Does this lead to no ATP production by F0F1-...

02 May 2013 9,457 1 View

Why is acidified methanol solution preferred to other solutions for the separation of metabolites? Is this related to partition coefficient?

Anthocyanin and Flavanoids are useful secondary metabolites from microbes and plants. These are separated using various solvents, especially acidified methanol solution. This is an industrial...

10 April 2013 8,313 1 View

Can anyone provide the specifications of nutrient medium for the growth of Nostoc , Phytoplankton and Macroalga?

Growth of these compounds can help us to collect useful enzymes by exposing them to UV radiation.

12 October 2012 1,044 5 View