Antimycin A inhibits ETC at the "cytochrome b" site in complex 3 . This regulation leads to the complete halt of electron transport in mitochondria . Does this lead to no ATP production by F0F1- ATPase (ATP synthase) ?
See the driving force behind the ATP synthesis during electron transport chain is called "proton motive force", which is basically developed due to the transport of H+ ions into the inter membrane space, while the ETC is happeneing. a total of 6 protons will be transported into the IMS during ETC. as a result, a proton gradient is established, with higher conc. of protons in the IMS. these protons will come back into the lumen through the F0F1 atpase. while the protons are moving through the atpase, this being the worlds smallest motor, rotates and uses that energy to add inorganic phosphate to ADP to generate ATP.
Now antimycin A, by inhibiting cytochrome B prevents the proton transport, as a result, the PMF is not developed in the first place. ie., there is no proton gradient at all as a result, ATP synthesis is blocked.