I am working on differentiating iPSCs to macrophages through EB method. in the last stage, we treat the developed monocytes with 100ng of m-CSF and 50ng IL-3 for 10 days in gelatin-coated dishes. but after day 10, the rest of the cells remain suspended and a very few number has attached.

1st photo is for monocytes in macrophage differentiating medium

2nd photo is for attached macrophages at day 10 after collecting suspended cells.

More Ahmed Salah Hassan's questions See All
Similar questions and discussions