I am working on differentiating iPSCs to macrophages through EB method. in the last stage, we treat the developed monocytes with 100ng of m-CSF and 50ng IL-3 for 10 days in gelatin-coated dishes. but after day 10, the rest of the cells remain suspended and a very few number has attached.
1st photo is for monocytes in macrophage differentiating medium
2nd photo is for attached macrophages at day 10 after collecting suspended cells.