Ahmed Salah Hassan

3 Questions 2 Answers 0 Followers

Questions related from Ahmed Salah Hassan

How to improve low A260/A230 ratio in RNA samples isolated from whole blood by QIAmp blood RNA mini kit from Qiagen?

I am using Qiagen Blood RNA Mini kit to isolate RNA from whole blood.. and the A260/A230 is quite low, even below 1. My main downstream application is RNA-seq. and I am not sure if this low ratio...

18 January 2024 9,944 0 View

How can I get an M0 macrophage from IPSC derived monocytes?

I am working in deriving different subtypes of macrophages from ipsc and thp-1, and compare between them

13 November 2019 3,930 2 View

Why ipsc-monocytes do not attached to gelatin dishes after 10 days treatment with RPMI supplmented with m-CSF and IL-3?

I am working on differentiating iPSCs to macrophages through EB method. in the last stage, we treat the developed monocytes with 100ng of m-CSF and 50ng IL-3 for 10 days in gelatin-coated dishes....

01 January 1970 4,780 0 View