Ahmed Salah Hassan

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Questions related from Ahmed Salah Hassan

How to improve low A260/A230 ratio in RNA samples isolated from whole blood by QIAmp blood RNA mini kit from Qiagen?

I am using Qiagen Blood RNA Mini kit to isolate RNA from whole blood.. and the A260/A230 is quite low, even below 1. My main downstream application is RNA-seq. and I am not sure if this low ratio...

18 January 2024 9,895 0 View

Why ipsc-monocytes do not attached to gelatin dishes after 10 days treatment with RPMI supplmented with m-CSF and IL-3?

I am working on differentiating iPSCs to macrophages through EB method. in the last stage, we treat the developed monocytes with 100ng of m-CSF and 50ng IL-3 for 10 days in gelatin-coated dishes....

01 January 1970 4,751 0 View