Iam extracting protein from red seaweed using sodium phosphate buffer, pH 7.0. I used different concentrations like 0.1M, 0.2M, 0.3M, 0.4M and 0.5M. After extracting protein, i found out that the protein% increased from 0.1M to 0.3M but it got reduced from 0.4M to 0.5M. What can be the reason behind this?