i have used beta mercaptoethanol for protein extraction.. But now how to remove it from the sample extract so that i can go for freeze drying..
Iam extracting proteins from seaweed. After extraction, lowry method showed the protein content in the sample. But when iam adding ammonium sulphate for protein precipitation, iam not observing...
20 September 2023 7,338 0 View
Iam extracting protein from red seaweed using sodium phosphate buffer, pH 7.0. I used different concentrations like 0.1M, 0.2M, 0.3M, 0.4M and 0.5M. After extracting protein, i found out that the...
06 July 2023 9,381 3 View
Iam working on seaweed protein.. Iam getting protein % of 62% through kjeldahl.. But when iam calculating Protein yield, its more than 100%.. Is it possible or what can be the reason behind this?...
12 June 2023 5,925 0 View
Iam working on seaweed protein. After extraction of protein, i have precipitated with ammonium sulphate and freeze dried the sample after dialysis. In kjeldahl iam getting good percentage of...
18 April 2023 7,611 0 View
I used 0.5% beta mercaptoethanol for extraction of protein from seaweed.. But for incorporation into food i should remove it from the sample.. Can anyone suggest me how to remove it?
29 March 2023 3,880 0 View
Iam unable to get any bands.. So it will be helpful if i know about alternatives..
20 March 2023 5,038 10 View
Tried with 7.5%, 10%, 12% and 15% gel percentages.. Protein concentration was checked through biuret and it is about 1.7mg/ml.. Used coomassie blue for staining (1hr) followed by destaining for...
19 March 2023 715 6 View
It is known that N conversion factors are used to determine protein content of a nitrogenous compound. My question is if we have an amino acid, for example arginine and its N content is 32%...
12 April 2017 6,307 3 View
I am trying to work out the nitrogen and energy content of a product. I only have info that nitrogen content in protein range from 13 to 19% with 16% taken as the average and hence conversion...
11 March 2017 6,231 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View