Chandini Inumala

5 Questions 10 Answers 0 Followers

Questions related from Chandini Inumala

Why increase in phosphate buffer concentration reducing the protein concentration of my sample?

Iam extracting protein from red seaweed using sodium phosphate buffer, pH 7.0. I used different concentrations like 0.1M, 0.2M, 0.3M, 0.4M and 0.5M. After extracting protein, i found out that the...

07 July 2023 9,215 3 View

How to remove beta mercaptoethanol from seaweed extract?

i have used beta mercaptoethanol for protein extraction.. But now how to remove it from the sample extract so that i can go for freeze drying..

05 April 2023 579 0 View

How to remove beta mercaptoethanol from seaweed extract?

I used 0.5% beta mercaptoethanol for extraction of protein from seaweed.. But for incorporation into food i should remove it from the sample.. Can anyone suggest me how to remove it?

30 March 2023 3,846 0 View

Are there any other alternatives to SDS PAGE to see the bands of proteins?

Iam unable to get any bands.. So it will be helpful if i know about alternatives..

21 March 2023 5,000 10 View

Iam trying SDS Page from last 5 months.. but not getting any bands.. My sample is seaweed.. What can be the reason for this?

Tried with 7.5%, 10%, 12% and 15% gel percentages.. Protein concentration was checked through biuret and it is about 1.7mg/ml.. Used coomassie blue for staining (1hr) followed by destaining for...

20 March 2023 644 6 View