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Questions related from Chandini Inumala
Iam extracting proteins from seaweed. After extraction, lowry method showed the protein content in the sample. But when iam adding ammonium sulphate for protein precipitation, iam not observing...
21 September 2023 7,317 0 View
Iam extracting protein from red seaweed using sodium phosphate buffer, pH 7.0. I used different concentrations like 0.1M, 0.2M, 0.3M, 0.4M and 0.5M. After extracting protein, i found out that the...
07 July 2023 9,253 3 View
Iam working on seaweed protein.. Iam getting protein % of 62% through kjeldahl.. But when iam calculating Protein yield, its more than 100%.. Is it possible or what can be the reason behind this?...
13 June 2023 5,880 0 View
Iam working on seaweed protein. After extraction of protein, i have precipitated with ammonium sulphate and freeze dried the sample after dialysis. In kjeldahl iam getting good percentage of...
19 April 2023 7,585 0 View
i have used beta mercaptoethanol for protein extraction.. But now how to remove it from the sample extract so that i can go for freeze drying..
05 April 2023 585 0 View
I used 0.5% beta mercaptoethanol for extraction of protein from seaweed.. But for incorporation into food i should remove it from the sample.. Can anyone suggest me how to remove it?
30 March 2023 3,855 0 View
Iam unable to get any bands.. So it will be helpful if i know about alternatives..
21 March 2023 5,007 10 View
Tried with 7.5%, 10%, 12% and 15% gel percentages.. Protein concentration was checked through biuret and it is about 1.7mg/ml.. Used coomassie blue for staining (1hr) followed by destaining for...
20 March 2023 655 6 View
