Why I obtain a dn/ds ratio that is obviously different between foreground and background in PAML but p-value is not significant?

13 June 2022 1 7K Report

When running clade model c in PAML, one branch that I think may experience elevated dN/dS is put in the foreground by me. After the run, the foreground has a dN/dS = 0.65 while the background is just 0.16. I think this level of difference is quite obvious. However, the p-value of comparison between clade model c using this partitioning and the null model M2a_rel is always insignificant(around 0.5).

So, are there any explanations that can explain this situation?

Jochen Wilhelm

What is the assumed distribution model? What test is used? What transformation was applied? What is the sample size? And what are the concrete data (values)?

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