Rahul Mallick

4 Questions 10 Answers 0 Followers

Questions related from Rahul Mallick

Can anyone suggest suitable RNA immunoprecipitation (RIP) protocol from cell?

I would like to see RNA-protein interaction following transcription process inside the cell. I am wondering if there is any protocol similar to protein co-immunoprecipitation,which can bind the...

28 October 2020 3,244 3 View

What is the best way to retrieve all the genes predisposing to a disease?

I am working with PPI network of Alzheimer's disease (AD) and type II Diabetes Mellitus (DM) . But I am facing difficulties to retrieve all risk genes predisposing to AD and DM. Some literature...

01 March 2018 3,260 4 View

Why I can't see any DNA band on Agarose gel?

I have transformed my cloned plasmid DNA into bacteria and cultured bacteria. Then, I extracted DNA from bacteria and run in 0.5% agarose gel. But I am not seeing any DNA band except ladder. (In...

24 February 2017 2,719 14 View

Why do you need to permeabilize cells or tissues before incubating with the primary antibody? How do you do it practically?

Immunohistochemistry Immunocytocheistry

24 September 2016 812 5 View