How to remove water from plant extract??

Hello everyone, I hope these words find you well. I used 80% methanol (solvent) for seaweed's extraction. I have performed rotary evaporator extraction in order to evaporate methanol but there is...

16 October 2020 5,598 8 View

How can we check the real content of calcium in the protein samples?

03 August 2020 4,309 3 View

Capability of MCNP6 in Muon tracking?

Is MCNP6 able to simulate galactic cosmic ray specifically Muons ? if yes does it have any energy or cross section restrictions?

16 June 2020 9,049 3 View

Can anyone provide or suggest some reliable articles on second order SEM modelling using SmartPLS.?

Hi everyone. I need some article to understand the second-order SEM modelling using PLS. If anyone can suggest or can share I shall be highly grateful.

03 March 2020 8,605 6 View

Help me construct a research topic on : open drug market and rising spate of drug abuse?

i need assistance on to construct a better research topic on the role of drug market on rising spate of drug abuse.

28 February 2020 8,251 3 View

Can I use an E-Beam patterned Photoresist as a mask for UV photoresist exposure?

Dear Seniors, I am wondering, if I could expose my pattern in E-beam resist (Suggest me one for this purpose) which is on top of UV photoresist (AZ some suitable one). Then I used this written...

21 February 2020 9,650 1 View

Assessment of air microflora in the microbiology Laboratory?

To assess the air microflora in indoor air of laboratories

14 January 2020 2,006 4 View

Which international universities hold the Digital Transformation DBA course?

Hi dears, I want to learn more about Digital Transformation and its impact on Business model, strategy, marketing, agility and ... .I want to apply for online course at universities; is there any...

24 December 2019 3,817 11 View

What techniques are involved in partitioning Directed Acyclic Graphs?

For a large DAG graph what technique can we employ to divide such a large graph into sub DAGs

16 December 2019 9,192 2 View

How to select rats/mice for in vivo studies?

I hope everyone is doing great. Kindly spare some time and help me in resolving my issue. How can we select animal for our in vivo study? what are the basis of selection? For instance, I am...

10 November 2019 3,769 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View