gMSCs were seeded on PCL/HAp scaffolds to see their adhesion and distribution properties on it. But even after 2 weeks of incubation, the cells are forming globular morphology rather than flat or spindle shape, that too single-single cells. No interactions were observed in between the cells, no processes. Cells are live proliferation also happening but morphology same.
Dear researcher
Check the porosity of your scaffold it is too important for attachment of cells
Hi Archana Rajendran
The attachment of cells on the scaffold will depend on hydrophilicity and surface roughness. You can improve the adhesion of cells on the scaffold by dipping in complete media/FBS before cell seeding or coating it with gelatin.
Jijo Thomas Thank you so much for the response. Yes, I am pre-soaking the scaffolds in media before seeding the cells. The thing is in the hydrophobic scaffolds the cells are in normal morphology only and the one which shows the globular morphology is comparatively hydrophilic only. Maybe the rigidity of the scaffolds also changes their appearance??? need to see
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