Why fructose is eluted first compared to glucose during HPLC analysis when both have same molecular weight?

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Peshala Eranga Gunasekara

Dear Mamta Thakur

In HPLC, elution is depending on the polarity not on the size of the component. There fore one can elute first if its polarity differs from another even the both have same molecular weight.

Good luck

Meghit Boumediene Khaled

Yes I do agree with Peshala

It depends on polarity.

Mamta Thakur

Thank you @ Khaled and Peshala Sir.

Ahmed Belloufa

In addition to professors suggestions above, that means stationary phase attracts glucose when travelling the column strongly than the fructose molecules and/or the fructose having solubility degree in the mobile phase greater than of the glucose one. Even if glucose & fructose having the same raw parameters, structurally they very different.