Why Extracellular matix degarded in PBS buffer system at constant temperature 37 degree Celsius?

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Jan Voskuil

You need protease inhibitors and EDTA as well in your PBS. That will prevent degradation.

Richard T Pace

Jan, EDTA would cause cell detachment from calcium floculation and integrin release right?

Matrix metalloproteinases (MMPs) are calcium-dependent zinc-containing endopeptidases and it might be useful having them inhibited as well by adding EDTA.

Ok, thank you for clarifying