Mechanism behind the Degradation of ECM scaffold in 1X PBS.
You need protease inhibitors and EDTA as well in your PBS. That will prevent degradation.
Jan, EDTA would cause cell detachment from calcium floculation and integrin release right?
Matrix metalloproteinases (MMPs) are calcium-dependent zinc-containing endopeptidases and it might be useful having them inhibited as well by adding EDTA.
Ok, thank you for clarifying
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