So I recently made a qPCR standard curve, and got an amazing R^2 of 0.999, but the "efficiency" of the reaction was well outside the standard 90-105%. But I am curious - what does that matter if the assay can predict the C(t) near perfectly. Is that just a convention because it is easier to extrapolate from a standard curve? Or is efficiency important because when you have a narrow slope small amounts of variation can be huge differences in quantity since it is on a log scale. (I presume the latter, but would like to hear from people).

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