3 Questions 4 Answers 0 Followers
Questions related from Nicholas William Garcia
So I recently made a qPCR standard curve, and got an amazing R^2 of 0.999, but the "efficiency" of the reaction was well outside the standard 90-105%. But I am curious - what does that matter if...
03 March 2015 6,316 4 View
I've identified a novel splice variant, and want to develop an assay to distinguish it from the other known variant. The only region I can target is small (the variant has a different splice site...
02 February 2015 2,909 4 View
I've been trying to amplify 3' splice variants of a hormone receptor gene, and I initially had great success. However, now I cannot repeat my results. The first 3-4 PCR's ran great, but now I only...
07 July 2014 4,166 3 View
