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So I recently made a qPCR standard curve, and got an amazing R^2 of 0.999, but the "efficiency" of the reaction was well outside the standard 90-105%. But I am curious - what does that matter if...
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I've identified a novel splice variant, and want to develop an assay to distinguish it from the other known variant. The only region I can target is small (the variant has a different splice site...
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