This is an SDS-PAGE silver stained gel. The sample is from an FPLC fractions mixed with sample buffer (has SDS and DTT). I'm not sure why the lane has darker edges all the way through (vertically). Would anyone know what is causing this? Could this be from overloading the wells?
It looks like the effect that you will get if the wells leak and some of your sample runs in the space between the gel and the backing plate so forms a smear and not bands. Maybe tightening the clamps that hold the gen and glass plates together might help
Looks more like a voltage issue during running, sometimes I have observed such band when the buffer in the inner tank leaked out resulting in improper electric field throughout the run. When lanes get overload you would not see such clean sharp bands for the low intensity proteins and it would be a whole merged lane.
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