Razan Alshamali

5 Questions 2 Answers 0 Followers

Questions related from Razan Alshamali

Can I use the transwell Boyden Assay for the movement of a purified protein?

I have a purified protein and I am wondering if it is possible for the protein to migrate from top chamber to lower chamber in the absence of any cells using the Boyden chamber assay. I've...

16 May 2024 4,369 0 View

Cell Lysate clogging FPLC column. What are some solutions?

I am trying to purify a cell lysate using a 1 mL His-trap column but the viscosity of the lysate is clogging up the column. What are some solutions to this?

22 February 2024 347 3 View

What's the longest time recommended for perfusing a heart on the Langendorff?

14 November 2023 4,467 2 View

Why does my Silver stained gel look like this?! Edges of the lane are dark (see attached)?

This is an SDS-PAGE silver stained gel. The sample is from an FPLC fractions mixed with sample buffer (has SDS and DTT). I'm not sure why the lane has darker edges all the way through...

24 August 2023 2,628 2 View

How do I prepare a Lyophilized FPLC fraction/elution for SDS-PAGE?

I have fractions collected of my protein from FPLC (liquid chromatography) and I am wondering how I am best able to load my samples on a SDS-PAGE gel after lyophilizing/freeze drying the...

23 March 2023 4,007 4 View