How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How should I calculate sample size when my research is about normal population? What is difference between sampling frame and sample size?

I am asking this question on behalf of my colleague who has to do thyroid's elasticity scan of normal population. This data will act as reference value in order to compare with diseased one. So,...

23 July 2020 8,303 2 View

Do you have any idea about the clinical significance of diffuse low density of thyroid gland ?

i noticed that the thyroid gland of some patients is quite low, similar to that of muscles. and the CT scan was described for various reasons, even including regular health checks. i tried pubmed,...

29 June 2020 3,238 6 View

How can we stabilize purified humanThyroid Stimulating Hormone (hTSH) in buffer, without the use of albumin or other large molecules?

We use purified hTSH (in PBS), but have issue with stability of the sample. Since the purified hormone is highly unstable the activity reduces drastically at room temperature. We had added BSA to...

06 July 2019 8,619 4 View

How can I improve Mask RCNN performance for nodule detection in Ultrasound thyroid images?

I am trying to use faster RCNN (in MATLAB) for nodule bounding box detection in a small thyroid Ultrasound dataset (only 233 images). Some nodules are big and some are very small. My question is...

05 June 2019 7,322 1 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

04 May 2019 6,718 1 View

Affordable source of Thyrotropin / bTSH?

I've been trying and failing to obtain cell-culture grade crude TSH (probably bovine) for propagation of laboratory thyroid cell lines (ie. FRTL5) - Merck / Sigma had a suitable product in their...

01 May 2019 1,335 1 View

Could you recommend some kits to measure mice T3/T4/TSH(thyroid hormnes)?

I wonder if there're some Elisa kits which have been identified to test mice T3/T4/TSH, I've searched R&D, and there're only T4 Kit.Or could I use the RIA machine used in clinical testing...

19 March 2019 598 1 View

What are the best supplements for thyroid gland and why?

Ive been researching this topic since a while and I would love to expand my knowledge in this area, Ive come across some studies claiming that selenium is effective in treating thyroid...

01 February 2019 4,003 18 View

Do you have Fluoride analysis of Psammoma bodies isolated from Cancers or could you have them analyzed now from stored specimens?

I am updating my earlier review on the role of Fluoride doped Hydroxyapatite in Cancer and my current focus is on Psammoma Bodies which have been found, and identifed as high risk, in a very wide...

31 January 2019 3,477 1 View

Hypothyroidism causes a secondary hypercholesterolemia. When the thyroid function is normalized with drugs, does hypercholesterolemia also normalize?

Hypothyroidism is criterion of exclusion in epidemiological studies. So, if the hypothyroidism is controlled, maybe is unnecessary be excluded these patients.

31 December 2018 2,354 1 View