I have HPLC agilent 1200. After 2 minutes from power it goes in shutdown mode. All the modules light became red except the degasser. I tried changing the column, and the mobile phase but gain nothing. All the connections are OK. Is there anyway to identify the problem?
Try locating all the leak sensors and check all of them are dry. If that doesn't work then might be your interface has a problem. You'll need an engineer to look at that. But its most likely a leak somewhere.
Do you have the handheld control module? One of the parameters that can be addressed through the module might be causing the system to automatically shutdown after 2 minutes. Also, check and make sure that all of the HPLC components in the system are setup correctly.
If you are using ChemStation software just take a look at the instrument logbook. One or more entries will appear telling you exactly which module recorded the error and what the error is. As noted earlier, a leak is the most common error (and every leak sensor needs to be 100% dry before proceeding, so fix any leaks found), but other common errors that will shut down a 1200-series instrument quickly would include a pump module which fails to find 'home', an injector module which fails its initialization, a detector lamp that is programmed to turn 'On', but fails to light. You need to go through each module, one-at-a-time to see if they power up properly and pass their initialization tests. These are very rugged systems so any failures are usually easy to spot.
I tightened the connections of the column then it finally worked. Many thanks
I had the same issue. The solution was to tighten all the connections. I believe the problematic tubing was the one connected to the 1200 Series Fluorescence Detector.
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