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What is the best mobile phase for washing c18 column before turning off the bump (methanol, acetonitrile, ...etc )? and what is the suitable flow and washing period?. I use HPLC Agilent 1200
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I have HPLC agilent 1200. After 2 minutes from power it goes in shutdown mode. All the modules light became red except the degasser. I tried changing the column, and the mobile phase but gain...
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Many references classified it as chemical treatment, however, I do not think that there is a chemical reaction.
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