11 November 2014 8 6K Report

Taking peptides (in CHCA matrix) as an example, why would most ions produced carries an 'overall net' +1 charge?

As a peptide must contain different combinations of acidic and basic amino acids (hence side chains are of different charges). How would all these charges add-up / behave (were they shielded?).

I am curious as in most database searches (e.g. Mascot), in essentially all cases, we set the peptide charge parameter to +1 and it works fine every time (i.e. most peaks in the spectrum could be matched in no problem).

Thanks.

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