12 Questions 36 Answers 0 Followers
Questions related from Kay Wong
Dear colleageus, I would like to seek your help: I have performed Western blot, 12% gel, loaded 30 microgram total proteins, for some cell lines. I used 2.5% milk powder for blocking, then apply...
04 April 2019 1,541 1 View
Dear colleagues, We are going to perform Lys-C digestion of proteins for proteomics experiments. The Lys-C comes in 2 AU format, but we are not familiarize with this Unit format. Conveniently, we...
04 April 2017 7,151 0 View
Dear colleagues, Following SCX, we've performed manual desalting of the individually-separated iTRAQ peptide fractions before LC-MS/MS. We used the commercially-available C18 Pipette Tips for the...
11 November 2016 8,991 6 View
Dear colleagues, We've tried to perform Co-immunoprecipitation on human cell line samples. After the Co-IP, we ran gels, excise individual bands of interest for MALDI-TOF/TOF protein...
03 March 2016 245 5 View
Dear colleagues, With access to only one Orbitrap instrument, we are conceiving 2 different LC systems for switching between proteomics (nano-UHPLC) and metabolomics (UHPLC) analyses once a...
06 June 2015 580 6 View
Dear Colleagues, I would like to seek your advice on another question: Lately, Our lab bought a commercial enzyme, and somehow we've run it on a SDS-PAGE gel. Intriguingly, we observed...
02 February 2015 8,209 8 View
Dear colleagues, I tried to analyze some phosphorylated peptides (around 8000 Da) in MALDI-TOF (positive linear mode). However, it's difficult to obtain a high resoultion, very sharp peak....
12 December 2014 2,111 5 View
Taking peptides (in CHCA matrix) as an example, why would most ions produced carries an 'overall net' +1 charge? As a peptide must contain different combinations of acidic and basic amino acids...
11 November 2014 5,901 8 View
Using the MaxQuant algorithm, I am analyzing some Orbitrap data from a label-free LC-MS/MS experiment. However, I encounter some difficulties in compiling the Experiment design template excel....
10 October 2014 6,398 8 View
To my knowledge, most such studies used correlation. But as mRNA gives rise to protein (which is a seemingly 'causative' relationship), Isn't that a regression analysis would otherwise be more...
09 September 2014 1,775 11 View
Hi, I have a real-time PCR assay plate that is ready to run (SYBR green dye, primers, enzyme, template...everything ready). But just prior the run, our instrument suddenly broke down (with an...
09 September 2014 7,449 8 View
After downloading the EST genomic database from NCBI GenBank (downloaded from ftp blast databases from NCBI as EST-others, at ftp://ftp.ncbi.nih.gov/blast/db/FASTA/)1. How could I convert the...
07 July 2014 715 2 View
