I am purifying fucose-containing-sulphated-polysaccharides/FCSP (potentially fucoidan) by anion exchange chromatography.
I then complex fucoidan with CTAB creating a fucoidan-CTAB salt and run it down the column. I then elute FCSP with various concentrations of EtOH.
Why does eluting FCSP with a concentration gradient of ethanol give rise to fractions with varying molecular weights and chemical composition?
Hello Grant,
I am not really sure of your question. Fucoidin is not a specific entity. It is a range of molecules of different sizes and different levels of sulfation. One would expect to see different fractions would show different MW, etc. just as chromatographically separating amylose will resolve the material into maltose, maltotriose, maltotetraose, etc.
Fucoidins can be pretty complex. I think that the attached article may help provide clarity.
I assume the ethanol gradient comprise CTAB conter ion to the anion excahnge resign. This would be normal anion exchange process. Ethanol has notheing to do with the eluction of the sulfate molecules unless hydrophic residue is involved with solubility. I think the starting material could be a complex molecules, rather than a homologues of the polysaccharides.
Simply because algal polysaccharides (as many other types) do have such a native distribution of molecular weight and do not have have regular chemical repeats.
Thank you Dr. Olechno and Dr. Yang for your insight and contributions. I will look into your suggestions. Greatly appreciated.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology