Why does DMSO become liquid with extended storage at -20C?

I noticed that drugs or reagents dissolved in DMSO and stored at -20C become liquid after a year or two of storage. Does anyone know why this is and if it affects the effectiveness of the...

30 September 2020 7,901 2 View

How do I deal with small sample size and nonnormality in SEMs (AMOS or R)?

Hello! I have a sample size of N = 70 at the moment, hoping to aproach 100. My Model has 21 parameters to be estimated. The Variables are all not normally distributed. Now the options that I know...

20 July 2020 9,849 6 View

What is the Full Spectrum of Neuroscience Research Techniques that Exist?

Hi! I graduated with a Neuroscience and Psychology BSc in 2016, and my final year project was a bench-lab in vitro study of omega-3 oils applied to fluorescently-stained cortical neurons. I...

26 May 2020 4,650 6 View

Which cell culture inserts do you use?

In our experiment we use TC treated 96-well plates from Eppendorf and coat our protein of interest. After coating we add monocytes and get our readout. Now, we want to add activated T cells to the...

02 March 2020 6,065 2 View

What is the most efficient way to exchange buffer in protein soltuions?

Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....

31 October 2019 793 4 View

Why does dynamic surface tension of gelatine solution increase before it decreases?

02 December 2018 949 3 View

Why are there a huge number of dead cells in my culture ?

Hello, I am culturing 293 cells for onward transfection. Unfortunately, I have observed that the cells are not multiplying as fast as they used to. Besides, i see several dead cells in the T25...

26 June 2018 4,274 5 View

Why does my SDS PAGE gel bubble and form a bell shape towards the bottom of the tank?

Hello, I am doing some SDS PAGE. Now, i am baffled by the fact that my gel bubbles at the bottom and there is a bell shape in the sample under separation, towards the bottom of the gel ( as...

15 May 2018 8,675 2 View

What is the best method to assess viability for adherent breast cancer cell lines?

For my project, I need to set up a viability method to see if inhibitors are responding. We currently don't know which method suits best and because there are so many we don't know which one we...

04 April 2018 2,737 3 View

What protocol could be implemented in analyzing for Total hydrocarbon content of soil using UV-Vis Spectrophotometer?

I need to determine the THC of crude oil polluted soil.

15 March 2018 7,557 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View