Please share your experience with glioblastoma cells. Thanks
I need to prepare a 40% KOH solution using KOH pellets and DH20 water.
01 February 2021 7,639 2 View
Is there any liquid formulation for mycotypha spore formulation to keep the spore as a liquid formula at room temparature?
03 August 2018 9,789 0 View
I am trying to build a business model based on my thesis which helps in providing the consumer insights about the advertisements for the marketers. Kindly share your experiences how you build a...
07 May 2017 1,134 7 View
As I am working on fungi secondary metabolite, I need to identify them through GC-MS or LC-MS. Previously i have gone for GC-MS but i didn't get results. So I need suggestions Please let me know...
13 March 2016 2,604 4 View
To analyze the terpenoids or compounds of fungal extracts which is a suitable way like UV Vis Spec,GCMS ,FTIR ? How to quantify the terpenoids from fungal extracts? If we use UV Vis Spec for...
29 January 2016 9,468 2 View
1) How to couple the transient thermal analysis into the mechanical analysis(structural analysis) to find the residual stresses 2) In ansys software 3) I have the transient thermal simulation...
06 December 2015 8,557 6 View
For example Temperature(c) conductivity(w/m k) Specific heat Density(kg/m3) 25 6.5 60 4470
06 April 2015 8,202 4 View
Fatty acid methyl esters percentage from GC-MS data
01 January 1970 5,979 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View