I want to prepare liposomes made of azolectin (sometimes spelled as asolectin).
I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge, discard the supernatant, dry) prior to adding chloroform.
What happens when I go through that washing procedure? Which fraction of azolectin is removed?
Kindly check this overview https://www.sciencedirect.com/topics/neuroscience/azolectin
Also check https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.883.2958&rep=rep1&type=pdf
Acetone is used for common cleaning of laboratory wares for a few reasons. Firstly it's because Acetone is a very good solvent, it is a very polar substance that dissolves almost all organic compounds, which is obviously criticial if you're cleaning. It is water miscible, so can be used in conjuction with water.
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