It is somewhat arbitrary but convenient value. You want to be sure to not use too much of the primary culture for inoculation so that you don't carry over waste products and signaling molecules from the old culture. And you don't want to dilute it too far back otherwise you have to wait longer for the secondary culture to grow. We often used a 100-fold when we wanted to use the secondary culture fairly quickly and 1000-fold when we wanted a bit of a delay or to capture the cells early in exponential growth. It also makes the math easy!
https://www.aatbio.com/resources/faq-frequently-asked-questions/What-are-the-differences-between-primary-and-secondary-cell-cultures
Kirankumar Nalla Thank you but i was asking about bacterial cultures.
The use of a 1% primary bacterial culture for a secondary culture is a common practice in microbiology and is done for several reasons:
In summary, the use of a 1% primary bacterial culture for a secondary culture is a common practice in microbiology that helps to reduce bacterial numbers, increase the probability of obtaining pure cultures, preserve the sample, and standardize the method.
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