Manideep Burra

5 Questions 3 Answers 0 Followers

Questions related from Manideep Burra

Why does E.coli bl21 cells are regrowing after lysis from a lytic protein expression ?

I have plasmid expressed a lytic protein in bl21 cells and did a growth kinetics and post induction there is reduction in turbidity which continued for three hours but post five the culture...

02 February 2025 2,335 2 View

Why am i Unable to cut the cloned insert ?

I have cloned one of my mutant gene constructs into pet28a and we confirmed the presence of insert on the vector backbone with colony pcr and plasmid pcr multiple times even expressed the protein...

06 March 2024 9,931 3 View

Which is the best commercially available kit for cell free protein synthesis (invitro translation) ?

out of different brands which one will be affordable and easy to use. we have options like new england bio labs, thermo scientific, sigma and promega. which one out of these is better to use.

31 July 2023 1,096 0 View

Why do we use 1 percent primary bacterial culture for secondary culture?

and reason for using primary and secondary bacterial cultures

06 December 2022 1,979 4 View

Why are gene expression analysis of clock genes done preferentially under free running condition of continuous light (LL) ?

Regarding clock genes expression in a. thaliana

21 April 2020 2,470 1 View