Under what conditions will we use the method of intraperitoneal injection of vaccination in mice? In streptococcus suis research, some studies vaccinated mice by intrapetitoneal injection? why?
to target gut mucosa and gut lymph nodes, IP route of vaccine administration is preferred.
yes, agreed with the above scholars answers ..IP injections are performed aiming that the vaccines will reach the near to the lymphoid tissues where immunological actions takes place.
On the other hand IM injections were targeting for bloodstream.
when applied to humans and pigs, can IP injection represent IM injection?
IP (Intraperitoneal) injection given into the peritoneum (body cavity) where as Intramuscular (IM) injection were employed in the dorsal or shoulder part of the fish/rabbit/pigs/human. So it differs (IP & IM)
Historically, IP injections have been given in mice for potency/immunogencity testing of vaccines. IP injections had been preferred to get a consistent high response due to gut lymphatics system, as discussed above and also doe to ease of injecting large volumes (0.5 ml). IP injection should not be a preferred route for evaluating vaccines, which are usually given by intramuscular (IM) route in humans, rarely subcutaneously (SC). To mimic the human response in mice, SC route should be preferred, because it is difficult to inject mice with accuracy by IM route due to very small amount of muscles, particularly in Balb/c mice. Another important point in injecting animals with adjuvanted vaccines, is not to dilute the vaccines before injection. Adjuvanted vaccines should be injected to animals undiluted in small fractions, such as 50 µl (usually 1/10th of human dose) or with a minimum dilution when necessary to get immune response in the middle of dose-response curve.
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