How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

Is there a way to convert MPN (most probable number) to CFU (colony forming unit)?

I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have one study which reports its microbial values in MPN. What should I do?

29 April 2020 662 5 View

How to clone 3 pieces?

Hello, I have to clone 3 pieces together: 1. 2 Pcr made- the inserts. One 4200bp (13 ng/microliter), one 460bp(21ng/microliter) 2. Vector 3800bp(13ngng/mictoliter). All pieces were cut with...

27 April 2020 809 3 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Direct viral RNA detection (RT_PCR)?

Is it possible, in your opinion, to detect RNA using RT-PCR but without RNA extraction? Considering for example a pharyngel swab sample, it is possbile to do a pre-treatment in order to preserve...

29 March 2020 7,576 5 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Parameters selection for HDBSCAN algorithm in Supervised setting ?

I would like to know if there is any kind of research on the parameter selection (minpoints) for the HDBSCAN algorithm, in the context of having a training set with only normal samples and a test...

06 March 2020 3,487 3 View

How can I solve convergence problems of a dynamic analysis of an earth dam with opensees?

Hello everyone! I'm trying to model a earth dam with opensees. To do TCL file, I used GiD ProblemTypes for 2D Slope Analysis Input File Generation. When I do dynamic analysis with small...

03 September 2019 2,508 2 View

Are ACO and PSO ranking based feature selection approaches?

Hello, I've just came across these two algorithms and I was wondering whether there are any available versions for ACO and PSO as ranking-based feature selection approaches? Any comment would be...

18 July 2019 4,528 8 View

How can I calculate the aerodynamic characteristics of horizontal tail in FLUENT?

22 May 2019 6,252 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View