I isolated primary liver cells from porcine by two step collagenase method. I am using RPMI 1640 medium for culturing the cells on collagen coated plates (collagen coating is done by myself). The cells are not attaching even it is 10 to 14 days old, but they are viable and forming clusters. Please suggest me any method to have cell attachment
Depending on what you want to do with the cells, you could try using Matrigel or a similar product. I found it to be very good for rat and human hepatocytes.
Thank you for the suggestions. The cells are not attached on the scaffolds also, I will try on nanospun mats. Cell isolation is carrried out immediately after taking organ to the lab and enzymes are also completely removed.
Thank you very much Sir. Now I am culturing with William's E medium from sigma. Can u send the papers for maintaining high yield and high viability. It will be helpful for me
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells