Janani Guru

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Questions related from Janani Guru

Why do primary liver cells isolated from porcine not attaching to the plate?

I isolated primary liver cells from porcine by two step collagenase method. I am using RPMI 1640 medium for culturing the cells on collagen coated plates (collagen coating is done by myself). The...

10 October 2015 4,785 3 View

Why are the decellularized samples showing high GAG content than in the native tissue?

I did GAG estimation for native tissues and decellularized samples by DMMB method. Decellularized samples are showing high content of GAG protein than in the native tissue. Is the DMMB reagents...

10 October 2015 4,437 7 View