i am warping my plates with paper but still some plates are get wet or formation of vapor occur during autoclave.
High-pressure steam is required to reach all autoclaved material (inside bottles and your plates) for proper sterilisation. Water droplets occur during cooling and steam condensation on your material. Therefore you dont want to wrap your plates because it lowers autoclaving efficiency. You can either i) ignore that water ii) use advanced autoclave with regulated cooling & drying program iii) use specialised dryer / oven with dry heat after autoclaving.
Autoclave sterilizes supplies through high pressured steam. Try placing the plates in a container that is autoclavable, or you can dry them in a laboratory oven afterwards.
It is a ordinary thing beside the vapor presence. I always dry my autoclaved material overnight in room 55º C. Do not worry, probably your material is sterile.
i tried too many options to minimize wet conditions at autoclave but i failed. imo best think sterilize at oven at 180C 2 hours.
the way that you are trying to autoclave ur media is wrong. why do you sterilize your media inside plates?!!!! usually people prepare their media inside a bottle then pour it into sterilized petri-dishes. During pouring, you should put plates on top of each other to avoid vapor inside petri-dishes. easy!
About Mr. Turkel suggestion, where you get this? nobody sterilize a media at 180C, at that temperature, all your organic substances such as sugars, amino acids ... will be destroyed surely.
meanwhile
If you just autoclaving empty petri dishes, you should use oven (dry sterilization) not autoclave (wet sterilization).
mr. reza khakvar i am talking about empty plates not about media . after autoclave am pouring my media in plates, my question is after autoclave vapor is form in my empty plates so how to get rid from vapor?
At the end of my previous massage I said that if you just atuoclaving empty ones, you should use oven not autoclave, 150C for 4 hours
hello, this is a question for Yeni Khairina . That protocol for drying wet petri dishes (put intro dry oven 80°c for 1 day) ¿where you see that step? is a normalaized protocol? (for example OMS, NHS UK, ISO etc). Thanks
Sergio Sepulveda that is a common protocol in our lab. we confirmed that most the glass wear completely dry after 1 day in oven.
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