After a special treatment of mice with chronic liver disease, I see a significant increase of monocyte-derived macrophages in the liver along with increased fibrosis and compensatory proliferation.
I stained liver section for Cl.Casp3 (Apoptosis) and observe that these monocytes (besides hepatocytes) are also apoptotic.
Can anyone give me hints why these monocytes undergo apoptosis inside the liver? Don't they support the inflammatory milieu?
Thanks!!
Hi Max, how do you distinguish between endogenous liver macrophages and monocyte derived macrophages? Are they labeled?
If so, are they in lysosomal structures or endocytic vacuoles?
Thank you both for your answers!
@wy Do you have an publication for me indicating the life-span of monocytes in mice?
@SS Normally we perform FACS analysis, gating LY6C+/CD11b+/F4/80+ cells as monocyte-derived macrophages. I did not yet check for lysosomal structures or endocytic vacuoles.
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